Physicochemical characterization and bioactivity assessment of chitosan-PEO blends
The work consists on the chemical, mechanical, morphological and biological characterization of polymer thin films. Films are produced by electrodeposition of chitosan-PEO blend in 4 different ratio (100:0, 80:20, 50:50 and 20:80) in order to modulate the elastic modulus and nanohardness. The chemical characterization is carried out by Fourier Transform Infrared spectroscopy (FTIR) and Raman spectroscopy. Nanomechanical properties will be assessed by Atomic Force Microscope (AFM), which in parallel with Confocal Raman Imaging, will provide 3-dimensional images of surface nanotopography. Cell cultures (human-osteblastic cells SaOS-2) will evaluate the bioactive properties of the chitosan-PEO films. In particular, for each category of substrates, staining with DAPI, Ki-67, rhodamine and vinculin will permit to assess cell growth and division, cytoskeletal rearrangement and establishment of focal adhesions.